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Experience: Works
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Plasmid

Part:BBa_K112246:Design

Designed by: Molly Allen   Group: iGEM08_UC_Berkeley   (2008-10-28)


{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3854
    Illegal PstI site found at 881
    Illegal PstI site found at 5837
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4696
    Illegal PstI site found at 881
    Illegal PstI site found at 5837
    Illegal NotI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 212
    Illegal XhoI site found at 5877
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3854
    Illegal PstI site found at 881
    Illegal PstI site found at 5837
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3854
    Illegal PstI site found at 881
    Illegal PstI site found at 5837
    Illegal AgeI site found at 4286
    Illegal AgeI site found at 4610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 762
    Illegal BsaI.rc site found at 4978


Design Notes

?


Source

pAH57 plasmid

References